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Quantitative real-time PCR detection of Zika virus and evaluation with field-caught Mosquitoes
dc.contributor.author | Faye, Oumar | |
dc.contributor.author | Faye, Ousmane | |
dc.contributor.author | Diallo, Diawo | |
dc.contributor.author | et al. | |
dc.date.accessioned | 2023-01-13T14:45:32Z | |
dc.date.available | 2023-01-13T14:45:32Z | |
dc.date.issued | 2014 | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016539/ | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12663/3341 | |
dc.description.abstract | Zika virus (ZIKV), a mosquito borne flavivirus is a pathogen affecting humans in Asia and Africa. ZIKV infection diagnosis relies on serology–which is challenging due to cross-reactions with other flaviviruses and/or absence or low titer of IgM and IgG antibodies at early phase of infection- virus isolation, which is labor intensive, time consuming and requires appropriate containment. Therefore, real-time RT-PCR (rRT-PCR) is an appealing option as a rapid, sensitive and specific method for detection of ZIKV in the early stage of infection. So far, only one rRT-PCR assay has been described in the context of the outbreak in Micronesia in 2007. In this study, we described a one step rRT-PCR for ZIKV which can detect a wider genetic diversity of ZIKV isolates from Asia and Africa. | en_US |
dc.language | English | en_US |
dc.subject | Zika Research Project | en_US |
dc.subject | Zika Virus | en_US |
dc.subject | RT-PCR | en_US |
dc.title | Quantitative real-time PCR detection of Zika virus and evaluation with field-caught Mosquitoes | en_US |
eihealth.country | Others | en_US |
eihealth.category | Epidemiology and epidemiological studies | en_US |
eihealth.type | Research protocol information | en_US |
eihealth.maincategory | Save Lives / Salvar Vidas | en_US |
dc.relation.ispartofjournal | Virology Journal | en_US |
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