dc.contributor.author | Gadea, Gilles | |
dc.contributor.author | Bos, Sandra | |
dc.contributor.author | Krejbich-Trotot, Pascale | |
dc.contributor.author | et al. | |
dc.date.accessioned | 2022-05-19T01:55:44Z | |
dc.date.available | 2022-05-19T01:55:44Z | |
dc.date.issued | 2016-07 | |
dc.identifier.uri | https://pubmed.ncbi.nlm.nih.gov/27471954/ | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12663/2733 | |
dc.description.abstract | Zika virus (ZIKV) infection is a major public health problem with severe human congenital and neurological anomalies. The screening of anti-ZIKV compounds and neutralizing antibodies needs reliable and rapid virus-based assays. Here, we described a convenient method leading to the rapid production of molecular clones of ZIKV. To generate a molecular clone of ZIKV strain MR766(NIID), the viral genome was directly assembled into Vero cells after introduction of four overlapping synthetic fragments that cover the full-length genomic RNA sequence. Such strategy has allowed the production of a recombinant ZIKV expressing the GFP reporter gene that is stable over two culturing rounds on Vero cells. Our data demonstrate that the ZIKV reporter virus is a very reliable GFP-based tool for analyzing viral growth and measuring the neutralizing antibody as well as rapid screening of antiviral effect of different classes of inhibitors. | en_US |
dc.language | English | en_US |
dc.subject | Zika Research Project | en_US |
dc.subject | Zika Virus | en_US |
dc.subject | Arbovirus | en_US |
dc.subject | Communicable Diseases, Emerging | en_US |
dc.title | A robust method for the rapid generation of recombinant Zika virus expressing the GFP reporter gene | en_US |
eihealth.country | Others | en_US |
eihealth.category | Epidemiology and epidemiological studies | en_US |
eihealth.type | Research protocol information | en_US |
eihealth.maincategory | Protect Health Care Workers / Proteger la Salud de los Trabajadores | en_US |
dc.relation.ispartofjournal | Virology | en_US |