Mostrar el registro sencillo del ítem
Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients
dc.contributor.author | Yu, Fengting et al. | |
dc.date.accessioned | 2020-12-17T20:53:44Z | |
dc.date.available | 2020-12-17T20:53:44Z | |
dc.date.issued | 2020-12-17 | |
dc.identifier.uri | https://doi.org/10.1093/cid/ciaa345 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12663/2420 | |
dc.description.abstract | Background: Coronavirus disease 2019 (COVID-19) has become a public health emergency. The widely used reverse transcription–polymerase chain reaction (RT-PCR) method has limitations for clinical diagnosis and treatment. Methods: A total of 323 samples from 76 COVID-19–confirmed patients were analyzed by droplet digital PCR (ddPCR) and RT-PCR based 2 target genes (ORF1ab and N). Nasal swabs, throat swabs, sputum, blood, and urine were collected. Clinical and imaging data were obtained for clinical staging. Results: In 95 samples that tested positive by both methods, the cycle threshold (Ct) of RT-PCR was highly correlated with the copy number of ddPCR (ORF1ab gene, R2 = 0.83; N gene, R2 = 0.87). Four (4/161) negative and 41 (41/67) single-gene positive samples tested by RT-PCR were positive according to ddPCR with viral loads ranging from 11.1 to 123.2 copies/test. The viral load of respiratory samples was then compared and the average viral load in sputum (17 429 ± 6920 copies/test) was found to be significantly higher than in throat swabs (2552 ± 1965 copies/test, P < .001) and nasal swabs (651 ± 501 copies/test, P < .001). Furthermore, the viral loads in the early and progressive stages were significantly higher than that in the recovery stage (46 800 ± 17 272 vs 1252 ± 1027, P < .001) analyzed by sputum samples. Conclusions: Quantitative monitoring of viral load in lower respiratory tract samples helps to evaluate disease progression, especially in cases of low viral load. | en_US |
dc.language | English | en_US |
dc.subject | COVID-19 | en_US |
dc.subject | Coronavirus | en_US |
dc.subject | Coronavirus Infections | en_US |
dc.subject | Infectious Diseases | en_US |
dc.subject | Betacoronavirus | en_US |
dc.subject | SARS-CoV-2 | en_US |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject | Viral Load | en_US |
dc.title | Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients | en_US |
eihealth.country | Others | en_US |
eihealth.category | Virus: natural history, transmission and diagnostics | en_US |
eihealth.type | Published Article | en_US |
eihealth.maincategory | Save Lives / Salvar Vidas | en_US |
dc.relation.ispartofjournal | Clinical Infectious Diseases | en_US |
Ficheros en el ítem
Ficheros | Tamaño | Formato | Ver |
---|---|---|---|
No hay ficheros asociados a este ítem. |