ListarTechnical documents and research evidence on COVID-19 por tema "Real-Time Polymerase Chain Reaction"
Mostrando ítems 1-15 de 15
-
Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA
(2020-05-19)To circumvent the limited availability of RNA extraction reagents, we aimed to develop a protocol for direct RT-qPCR to detect SARS-CoV-2 in nasopharyngeal swabs without RNA extraction. Nasopharyngeal specimens positive ... -
Effect of Heat inactivation on Real-Time Reverse Transcription PCR of the SARS-COV-2 Detection
(2020-05-28)Background: Real-time reverse transcription PCR (rRT-PCR) is commonly used to diagnose SARS-CoV-2 infection. Heat inactivation prior to nucleic acid isolation may allow safe testing, while the effects of heat inactivation ... -
Efficient Prevalence Estimation and Population Screening for SARS-CoV-2
(2020-05-22)The testing capacity for SARS-CoV-2 using RT-PCR analyses can be substantially increased by using pooling techniques. Here we discuss two important consequences of this. First, prevalence estimation can be done with a very ... -
False-Negative results of initial RT-PCR assays for Covid-19: a systematic review
(2020-04-21)Background: Cases with negative reverse transcription-polymerase chain reaction (RT-PCR) results at initial testing for suspicion of SARS-CoV-2 infection, and found to be positive in a subsequent test, are considered as ... -
How best to use limited tests? Improving COVID-19 surveillance in long-term care
(2020-04-22)Background: Long-term care facilities (LTCFs) are particularly vulnerable to nosocomial outbreaks of coronavirus disease 2019 (COVID-19), with high rates of transmission and mortality. Timely epidemiological surveillance ... -
Increased PCR screening capacity using a multi-replicate pooling scheme
(2020-04-22)Effective public health response to viral outbreaks such as SARS-CoV-2 is often informed by real-time PCR screening of large populations. Pooling samples can increase screening capacity. However, when a traditional pool ... -
Increased PCR screening capacity using a multi-replicate pooling scheme
(2020-04-22)Effective public health response to viral outbreaks such as SARS-CoV-2 is often informed by real-time PCR screening of large populations. Pooling samples can increase screening capacity. However, when a traditional pool ... -
Large-scale implementation of pooled RNA-extraction and RT-PCR for SARS-CoV-2 detection
(2020-05-07)Testing for active SARS-CoV-2 infection is a fundamental tool in the public health measures taken to control the COVID-19 pandemic. Due to the overwhelming use of SARS-CoV-2 RT-PCR tests worldwide, availability of test ... -
Rapid identification of SARS-CoV-2-infected patients at the emergency department using routine testing
(2020-04-24)Background: The novel coronavirus disease 19 (COVID-19), caused by SARS-CoV-2, spreads rapidly across the world. The exponential increase in the number of cases has resulted in overcrowding of emergency departments (ED). ... -
Saliva is more sensitive for SARS-CoV-2 detection in COVID-19 patients than nasopharyngeal swabs
(2020-04-22)Rapid and accurate SARS-CoV-2 diagnostic testing is essential for controlling the ongoing COVID-19 pandemic. The current gold standard for COVID-19 diagnosis is real-time RT-PCR detection of SARS-CoV-2 from nasopharyngeal ... -
Screening for SARS-CoV-2 infections with colorimetric RT-LAMP and LAMP sequencing
(2020-05-09)The SARS-CoV-2 (COVID-19) pandemic poses a significant public-health problem. In order to control the pandemic, rapid tests for detecting existing infections and assessing virus spread are critical. Approaches to detect ... -
Sensitivity and specificity of a rapid test for assessment of exposure to SARS-CoV-2 in a community-based setting in Brazil
(2020-05-10)Background: While the recommended laboratory diagnosis of COVID-19 is a molecular based assay, population-based studies to determine the prevalence of COVID-19 usually use serological assays. Objective: To evaluate the ... -
Test performance evaluation of SARS-CoV-2 serological assays
(2020-05-17)Background Serological tests are crucial tools for assessments of SARS-CoV-2 exposure, infection and potential immunity. Their appropriate use and interpretation require accurate assay performance data. Method We conducted ... -
The use of convalescent plasma for pediatric patients with SARS-CoV-2: A systematic literature review
(2020-12-23)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes coronavirus disease 2019 (COVID-19), a severe illness leading to pneumonia, multiorgan failure, and death. With this study, we performed ... -
Validation of an extraction-free RT-PCR protocol for detection of SARS-CoV2 RNA
(2020-05-01)In light of supply chain failures for reagents and consumables needed for purification of nucleic acid for detection of SARS-CoV-2 RNA by RT-PCR, we aim to verify the performance and utility of a non-extraction protocol ...