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dc.contributor.authorliu, yanxia et al.
dc.date.accessioned2020-06-16T17:14:20Z
dc.date.available2020-06-16T17:14:20Z
dc.date.issued2020-05-28
dc.identifier.urihttps://doi.org/10.1101/2020.05.19.20101469en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12663/1759
dc.description.abstractBackground: Real-time reverse transcription PCR (rRT-PCR) is commonly used to diagnose SARS-CoV-2 infection. Heat inactivation prior to nucleic acid isolation may allow safe testing, while the effects of heat inactivation on SARS-CoV-2 rRT-PCR detection result need to be determined. Methods: 14 positive nasopharyngeal swab specimens were inactivated at 56°C for 30min, 56°C for 60min, 60°C for 30min, 60°C for 75min, and 100°C for 10min, and another 2 positive nasopharyngeal swab specimens were also inactivated at 100°C for 10min, 100°C for 30min, 100°C for 60min, after which the samples were isolated and detected by rRT-PCR. Results: All 14 heat treated samples remained positive. The range of threshold cycle (Ct) values observed when detecting ORF1a/b was 27.228-34.011 in heat-treated samples, while 25.281-34.861 in unheated samples, and the range of threshold cycle (Ct) values observed at the time of detecting N was 25.777-33.351 in heat-treated samples, while 24.1615-35.433 in unheated samples, on basis of which it showed no statistical difference otherwise a good correlation of Ct values between the heat-inactivated samples and the untreated samples. However, the 2 samples inactivated at 100°C 30min, 100°C 60min turned into negative. Conclusions: Heat inactivation at 56°C for 30min, 56°C for 60min, 60°C for 30min, 60°C for 75min, and 100°C for 10min shall not affect the detection results of Real-Time Reverse Transcription PCR of the SARS-COV2. Furthermore, it is recommended to inactive nasopharyngeal swab specimens 10min at 100°C before RNA extraction in consideration of efficiency and reliable results. Key Words: SARS-CoV-2, Heat Inactivation, rRT-PCR, Comparison.en_US
dc.languageEnglishen_US
dc.subjectCOVID-19en_US
dc.subjectCoronavirusen_US
dc.subjectInfectious Diseasesen_US
dc.subjectBetacoronavirusen_US
dc.subjectReal-Time Polymerase Chain Reactionen_US
dc.subjectCoronavirus Infectionsen_US
dc.titleEffect of Heat inactivation on Real-Time Reverse Transcription PCR of the SARS-COV-2 Detectionen_US
eihealth.countryGlobal (WHO/OMS)en_US
eihealth.categoryVirus: natural history, transmission and diagnosticsen_US
eihealth.typePublished Articleen_US
eihealth.maincategorySlow Spread / Reducir la Dispersiónen_US
dc.relation.ispartofjournalmedRxiven_US


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