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dc.contributor.authorArumugam, Arunkumar et al.
dc.date.accessioned2020-05-28T18:26:50Z
dc.date.available2020-05-28T18:26:50Z
dc.date.issued2020-04-30
dc.identifier.urihttps://doi.org/10.1101/2020.04.29.069591en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12663/1631
dc.description.abstractQuantitative reverse transcription polymerase chain reaction (RT-qPCR) assay is the gold standard recommended to test for acute SARS-CoV-2 infection. It has been used by the Centers for Disease Control and Prevention (CDC) and several other companies in their Emergency Use Authorization (EUA) assays. RT-qPCR requires expensive equipment such as RNA isolation instruments and real-time PCR thermal cyclers, which are not available in many low resource settings and developing countries. As a pandemic, COVID-19 has quickly spread to the rest of the world. Many underdeveloped and developing counties do not have the means for fast and accurate COVID-19 detection to control this outbreak. Using COVID-19 positive clinical specimens, we demonstrated that RT-PCR assays can be performed in as little as 12 minutes using untreated samples, heat-inactivated samples, or extracted RNA templates. Rapid RT-PCR was achieved using thin-walled PCR tubes and a setup including sous vide immersion heaters/circulators. Our data suggest that rapid RT-PCR can be implemented for sensitive and specific molecular diagnosis of COVID-19 in situations where sophisticated laboratory instruments are not available.en_US
dc.languageEnglishen_US
dc.subjectCOVID-19en_US
dc.subjectCoronavirusen_US
dc.subjectInfectious Diseasesen_US
dc.subjectSARS-CoVen_US
dc.subjectBetacoronavirusen_US
dc.subjectReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleA Rapid COVID-19 RT-PCR Detection Assay for Low Resource Settingsen_US
eihealth.countryGlobal (WHO/OMS)en_US
eihealth.categoryVirus: natural history, transmission and diagnosticsen_US
eihealth.typePublished Articleen_US
eihealth.maincategorySlow Spread / Reducir la Dispersiónen_US
dc.relation.ispartofjournalmedRxiven_US


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