dc.contributor.author | Brown, Julianne R et al. | |
dc.date.accessioned | 2020-05-28T13:27:04Z | |
dc.date.available | 2020-05-28T13:27:04Z | |
dc.date.issued | 2020-05-01 | |
dc.identifier.uri | https://doi.org/10.1101/2020.04.29.20085910 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12663/1624 | |
dc.description.abstract | In light of supply chain failures for reagents and consumables needed for purification of nucleic acid for detection of SARS-CoV-2 RNA by RT-PCR, we aim to verify the performance and utility of a non-extraction protocol for RT-PCR ("direct RT-PCR"). We report improved sensitivity compared to earlier reports of direct RT-PCR testing of swab samples, in particular at the lower limit of detection (sensitivity 93% overall; 100% for specimens with high to moderate viral titre, Ct <34; 81% for specimens with a low viral titre, Ct ≥34). Sensitivity is improved (from 90 to 93%) by testing in duplicate. We recommend swabs are re-suspended in water to minimise PCR inhibition. A cellular target is necessary to control for PCR inhibition and specimen quality. Direct RT-PCR is best suited to population level screening where results are not clinically actionable, however in the event of a critical supply chain failure direct RT-PCR is fit for purpose for the detection of SARS-CoV-2 infection. The results from our study offer front-line laboratories additional reagent options for performing extraction-free RT-PCR protocols. | en_US |
dc.language | English | en_US |
dc.subject | COVID-19 | en_US |
dc.subject | Coronavirus | en_US |
dc.subject | Infectious Diseases | en_US |
dc.subject | SARS-CoV | en_US |
dc.subject | Betacoronavirus | en_US |
dc.subject | Real-Time Polymerase Chain Reaction | en_US |
dc.title | Validation of an extraction-free RT-PCR protocol for detection of SARS-CoV2 RNA | en_US |
eihealth.country | Global (WHO/OMS) | en_US |
eihealth.category | Virus: natural history, transmission and diagnostics | en_US |
eihealth.type | Published Article | en_US |
eihealth.maincategory | Slow Spread / Reducir la Dispersión | en_US |
dc.relation.ispartofjournal | medRxiv | en_US |