Browsing by Subject "Polymerase Chain Reaction"
Now showing items 1-20 of 22
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Clinical and CT features of early stage patients with COVID-19: a retrospective analysis of imported cases in Shanghai, China
(2020-02-29)To the Editor: In December 2019, patients with novel corona virus disease 2019 (COVID-19) emerged in Wuhan, China. The pathogen analysis discovered a new type of coronavirus from infected airway epithelial cells, which was ... -
Clinical and epidemiologic profile of the initial COVID-19 patients at a tertiary care centre in India
(2020-04-10)COVID-19 has now become a pandemic. It has spread from Wuhan, China, in December 2019 to a large number of countries within three months. The objective of this work is to report the initial experience with epidemiologic ... -
Clinical and microbiological effect of a combination of hydroxychloroquine and azithromycin in 80 COVID-19 patients with at least a six-day follow up: A pilot observational study
(2020-03)Background: We need an effective treatment to cure COVID-19 patients and to decrease virus carriage duration. Methods: We conducted an uncontrolled, non-comparative, observational study in a cohort of 80 relatively mildly ... -
Clinical Characteristics of COVID-19 Patients With Digestive Symptoms in Hubei, China: A Descriptive, Cross-Sectional, Multicenter Study
(2020-04-14)OBJECTIVE: Since the outbreak of Coronavirus Disease 2019 (COVID-19) in December 2019, various digestive symptoms have been frequently reported in patients infected with the virus. In this study, we aimed to further ... -
Clinical decision support tool and rapid point-of-care platform for determining disease severity in patients with COVID-19
(2020-06-03)SARS-CoV-2 is the virus that causes coronavirus disease (COVID-19) which has reached pandemic levels resulting in significant morbidity and mortality affecting every inhabited continent. The large number of patients requiring ... -
A cohort study of 223 patients explores the clinical risk factors for the severity diagnosis of COVID-19
(2020-04-24)oronavirus Disease 2019 (COVID-19) has recently become a public emergency and a worldwide pandemic. The clinical symptoms of severe and non-severe patients vary, and the case-fatality rate (CFR) in severe COVID-19 patients ... -
A comparison of health care worker-collected foam and polyester nasal swabs in convalescent COVID-19 patients
(2020-05-01)Background: The exponential growth of COVID-19 cases and testing has created supply shortages at various points in the testing workflow. As of April 15, 2020 FDA recommendations only allowed for the use of nasopharyngeal, ... -
Comparison of SARS-CoV-2 detection in nasopharyngeal swab and saliva
(2020-05-24)We prospectively compared the efficacy of PCR detection of SARS-CoV-2 between paired nasopharyngeal and saliva samples in 76 patients including ten COVID-19 patients. The overall concordance rate of the virus detection ... -
Comparison of two commercial molecular tests and a laboratory-developed modification of the CDC 2019-nCOV RT-PCR assay for the qualitative detection of SARS-CoV-2 from upper respiratory tract specimens
(2020-05-06)We compared the ability of 2 commercial molecular amplification assays [RealTime SARS-CoV-2 on the m2000 (Abbott) and ID NOW™ COVID-19 (Abbott)] and a laboratory developed test [modified CDC 2019-nCoV RT-PCR assay with RNA ... -
Diagnostic power of chest CT for COVID-19: to screen or not to screen
(2020-05-24)Background: chest CT is increasingly used for COVID-19 screening in healthcare systems with limited SARS-CoV-2 PCR capacity. Its diagnostic value was supported by studies with methodological concerns and its use is ... -
False positives in reverse transcription PCR testing for SARS-CoV-2
(2020-05-20)Large-scale testing for SARS-CoV-2 by the reverse transcription polymerase chain reaction is a key part of the response to the COVID-19 pandemic, but little attention has been paid to the potential frequency and impacts ... -
Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction
(2014)A reverse transcription/polymerase chain reaction (RT/PCR) protocol for the rapid detection and identification of flaviviruses was developed using a set of universal oligonucleotide primers. These primers correspond to ... -
Is sleep apnoea a risk factor for Covid-19? Findings from a retrospective cohort study
(2020-05-18)Background: In the early phase of the coronavirus disease-19 (Covid-19) pandemic, Southwest Finland remained relatively spared. By the 3rd of May 2020, a total of 28 patients have been admitted to the Turku University ... -
Longitudinal laboratory testing tied to PCR diagnostics in COVID-19 patients reveals temporal evolution of distinctive coagulopathy signatures
(2020-05-23)Temporal inference from laboratory testing results and their triangulation with clinical outcomes as described in the associated unstructured text from the provider notes in the Electronic Health Record (EHR) is integral ... -
Occurrence and Timing of Subsequent SARS-CoV-2 RT-PCR Positivity Among Initially Negative Patients
(2020-05-08)Background: SARS-CoV-2 reverse transcriptase polymerase chain reaction (RT-PCR) testing remains the cornerstone of laboratory-based identification of patients with COVID-19. As the availability and speed of SARS-CoV-2 ... -
PCR positives: what do they mean?
(2020-09)Contents Is the PCR test sensitive enough?. page 2 Culturing a virus as reference test page 2 Does a PCR “TRUE POSITIVE” mean INFECTIVITY OR VIRULENCE?. page 2 PCR true positives versus infectivity and virulence. page ... -
Prevalência do vírus Zika em doadores de sangue de um serviço de hemoterapia da Região Sul do Brasil
(2017)O vírus Zika (ZIKV) é um flavivírus responsável por causar uma arbovirose emergente no Brasil que é transmitido principalmente por picada de mosquitos infectados do gênero Aedes. Uma via de transmissão muito discutida ... -
Safety and Efficacy of Hydroxychloroquine in COVID-19: A Systematic Review and Meta-Analysis
(2020-08)Background: During the initial phases of the coronavirus disease 2019 (COVID-19) epidemic, there was an unfounded fervor surrounding the use of hydroxychloroquine (HCQ); however, recently, the Centers for Disease Control ... -
Sensitivity and specificity of a rapid test for assessment of exposure to SARS-CoV-2 in a community-based setting in Brazil
(2020-05-10)Background: While the recommended laboratory diagnosis of COVID-19 is a molecular based assay, population-based studies to determine the prevalence of COVID-19 usually use serological assays. Objective: To evaluate the ... -
Sensitivity assessment of SARS-CoV-2 PCR assays developed by WHO referral laboratories
(2020-05-04)The sensitivity of SARS-CoV-2 RT-PCR tests developed by Charite (Germany), HKU (Hong-Kong), China CDC (China), US CDC (United-States), and Institut Pasteur, Paris (France) was assessed on SARS-CoV-2 cell culture supernatants ...