dc.contributor.author | Arumugam, Arunkumar et al. | |
dc.date.accessioned | 2020-05-28T18:26:50Z | |
dc.date.available | 2020-05-28T18:26:50Z | |
dc.date.issued | 2020-04-30 | |
dc.identifier.uri | https://doi.org/10.1101/2020.04.29.069591 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12663/1631 | |
dc.description.abstract | Quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay is the gold standard recommended to test for acute SARS-CoV-2 infection. It has been used by the Centers for Disease Control and Prevention (CDC) and several other companies in their Emergency Use Authorization (EUA) assays. RT-qPCR requires expensive equipment such as RNA isolation instruments and real-time PCR thermal cyclers, which are not available in many low resource settings and developing countries. As a pandemic, COVID-19 has quickly spread to the rest of the world. Many underdeveloped and developing counties do not have the means for fast and accurate COVID-19 detection to control this outbreak. Using COVID-19 positive clinical specimens, we demonstrated that RT-PCR assays can be performed in as little as 12 minutes using untreated samples, heat-inactivated samples, or extracted RNA templates. Rapid RT-PCR was achieved using thin-walled PCR tubes and a setup including sous vide immersion heaters/circulators. Our data suggest that rapid RT-PCR can be implemented for sensitive and specific molecular diagnosis of COVID-19 in situations where sophisticated laboratory instruments are not available. | en_US |
dc.language | English | en_US |
dc.subject | COVID-19 | en_US |
dc.subject | Coronavirus | en_US |
dc.subject | Infectious Diseases | en_US |
dc.subject | SARS-CoV | en_US |
dc.subject | Betacoronavirus | en_US |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.title | A Rapid COVID-19 RT-PCR Detection Assay for Low Resource Settings | en_US |
eihealth.country | Global (WHO/OMS) | en_US |
eihealth.category | Virus: natural history, transmission and diagnostics | en_US |
eihealth.type | Published Article | en_US |
eihealth.maincategory | Slow Spread / Reducir la Dispersión | en_US |
dc.relation.ispartofjournal | medRxiv | en_US |